FAO Nutrition Meetings
Resort Series No. 44A
TOXICOLOGICAL EVALUATION OF SOME
FLAVOURING SUBSTANCES AND
NON-NUTRITIVE SWEETENING AGENTS
Geneva, 21-28 August 1967
The Eleventh Report of the Joint FAO/WHO Expert Committee on Food
Additives is published as FAO Nutrition Meetings Report Series,
1967, No. 44; Wld Hlth Org. techn. Rep. Ser., 1968, 383. This
Report contains general considerations, including the principles
adopted for the evaluation, and a summary of the results of the
evaluations of a number of food additives. Additional information,
such as biological data and a toxicological evaluation, considered at
that meeting, is to be found in this document.
Food and Agriculture Organization of the United Nations
World Health Organization
Chemical name Ethyl butanoate
Empirical formula C6H12O2
Structural formula CH3CH2CH2COOC2H5
Molecular weight 116.16
Definition Ethyl butyrate contains not less than 97
per cent. C6H12O2.
Description Ethyl butyrate may be prepared by the
esterification of normal butyric acid
with alcohol. It is a colourless liquid
with a fruity odour
This ester is hydrolysed by pancreatic lipase to ethyl alcohol
and butyric acid (Oppenheimer, 1925; Nishida, 1957).
Animal Route LD50 References
Rat oral 13 050 Jenner et al., 1964
Rat. In a feeding experiment on 15 males and 15 females for 12
weeks no adverse effect was noted at 14.4 mg/kg body-weight/day (Oser,
This compound hydrolyzes into normal constituents of food.
Evaluation is based on this and the short-term studies. Further
biochemical and metabolic studies are considered desirable.
Estimate of acceptable daily intake for man
Unconditional acceptance 0-15
Fassett, D. W. (1963) ln: Patty, F. A., Inductrial Hygiene and
Toxicology, Second Edition, interscience, New York & London
Jenner, P. M., Hagan, E. C., Taylor, J. M.., Cook, E. L. & Fitzhugh,
O. G. (1964) Fd Cosmet. Toxicol., 2, 327
Nishida, M. (1957) Yakugaku Zasshi, 77, 1141
Oppenheimer, C. (1925) Die Fermente und ihre Wirkungen, Leipzig, p.
Oser, B. L. (1967) Unpnblished report